CELL PROLIFERATION AS AN INDEX OF GROWTH IN CORALS; INCORPORATION OF ^3H-THYMIDINE
نویسندگان
چکیده
منابع مشابه
Incorporation of [3H]thymidine in mouse spleen.
Mice were given 4 injections of [H]thymidine at 2-hourly intervals and killed at times varying from 1 h to 12 days after the last injection. Autoradiographs of spleen, oesophagus and heart were prepared by the stripping-film technique and exposed for 21, 189 and 367 days. Groups of lymphoid cells surrounding trabeculae were heavily labelled, the decline in grain count suggesting division of the...
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As markers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous system. However, the effect of the incorporation of these molecules into DNA on cell proliferation, migration, differentiation, and function is not fully understo...
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Protein-calorie malnutrition or fasting leads to a readily detectable decrease in the rate of body growth and cellular proliferation, particularly in young animals (32, 33) . In contrast, disorders in cell and organ function have generally been more difficult to demonstrate, perhaps partly due to limitations in methodology (8, 16, 22) . Mitochondria are the primary energy source for the work of...
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Growth of HeLa S3 cells in monolayer cultures of Joklik's minimum essential medium, 5% fetal calf serum, and 2 mM glutamine in the presence of 10(-6) M dexamethasone results in an approximately 70% inhibition of exogenously added [3H]thymidine incorporation into DNA. In marked contrast, dexamethasone does not alter HeLa S3 cell growth rate under these conditions. The half-maximal inhibitory con...
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Epithelial cell proliferation was studied in the normal colonic mucosa of 5-week-old Sprague-Dawley rats, comparing [3H]thymidine incorporation (group 1) with two newer proliferation markers, bromodeoxyuridine (group 2) and proliferating cell nuclear antigen (group 3). Microautoradiography (group 1) or immunoperoxidase assays (groups 2 and 3) were carried out. Cells were counted for positive re...
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ژورنال
عنوان ژورنال: Publications of the Seto Marine Biological Laboratory
سال: 1973
ISSN: 0037-2870
DOI: 10.5134/175772